To be revised
The "ramp clamp" was so named to describe an experiment in which
the control voltage was swept with a linear increase
in voltage with time (ramp) from a resting level to a strong depolarized value. The
membrane current was plotted vs voltage directly and continuously rather
than plotting points for the peak and steady state currents-vs-voltage
for a step voltage clamp. For certain restricted rates of voltage sweep,
the ramp clamp current-voltage relationship resembled the form of
conventional I/V plots.
More precisely, a fast ramp was used to yield a plot resembling the sodium
current and a slow ramp to plot the potassium current (Moore, 1959).
Of course for other rates, the shape of the curve deviates sharply
because both the sodium and potasium conductances vary with time as well
as voltage and BOTH contribute to the current observed at any time. Furthermore
the results were contaminated with a constant capacitive current
flowing during the ramp phase. Nevertheless it was proposed as a useful
way to record data very quickly.
However, when Na channel kinetics are changed, for example, by:
- temperature changes
- certain drugs such as anesthtic agents
- changing Ca++ in the bath
the intrepretation of the records is difficult - `if not compromised to
the point of lack of uselessness.
xx needs revision here- Fishman did know of Ik at fast sweeps.
give fishman ref here
The most obvious failing with the ramp
clamp experiments on squid axons showed up when the sodium conductance was blocked by the
application of tetrodotoxin. While the inward
current disappeared for a fast ramp, an outward current persisted at
voltages above the sodium equilibrium potential! Analog
simulations of this conditions showed similar plots and revealed that
the apparent outward sodium current on a fast sweep was actually carried
by potassium!!